Matt Damon as Jason Bourne (Captain David Webb): an amnesiac who was previously a CIA assassin, Bourne is trying to escape Moscow police at the beginning of the film, shortly after killing a Russian assassin in an auto chase near the end of the previous film.
David Strathairn as Noah Vosen: a CIA Deputy Director, Vosen is the head of Operation Blackbriar, a renamed and upgraded Operation Treadstone.
Joan Allen as Pamela Landy: a CIA Deputy Director, Landy is working with Vosen to track and capture Bourne.
Julia Stiles as Nicky Parsons: formerly Bourne's Treadstone contact in Paris, she was assigned to Madrid after attempts to capture Bourne in Berlin and Russia failed.
Paddy Considine as Simon Ross: a British journalist who has been uncovering the secrets behind the origin of Operation Treadstone, and Bourne's past.
Albert Finney as Dr. Albert Hirsch: the CIA specialist who supervised Bourne's behavior modification training prior to Operation Treadstone.
Scott Glenn as Ezra Kramer: Director of the CIA.
Colin Stinton as Neal Daniels: CIA's Station Chief in Madrid.
Joey Ansah as Desh Bouksani: a Blackbriar assassin quartered in North Africa.
Edgar Ramirez as Paz: a Blackbriar assassin who is sent after Ross and Bourne.
Tom Gallop as Tom Cronin: Landy's deputy.
Corey Johnson as Wills: Vosen's deputy at Operation Blackbriar.
Sam Dang as Raymond Sykes: Deputy commander of CIA New York branch. Former field agent attached to Vosen.